ABSTRACT
OBJECTIVE:To establish the quality standard for Tongmai pill. METHODS:TLC was used for the qualitative iden-tification of Salvia miltiorrhiza,Angelica sinensis and Carthamus tinctorius. HPLC was used for the content determination of feru-lic acid. The column was Diamonsil C18 with mobile phase of acetonitrile-0.1% aqueous phosphoric acid (17∶83,V/V) at a flow rate of 1 ml/min,the detection wavelength was 316 nm,the column temperature was 30 ℃,and the injection volume was 10 μl.. RESULTS:The TLC of S. miltiorrhiza,A. sinensis and C. tinctorius showed clear spots and good separation. The linear range of ferulic acid was 8-80 μg/ml(r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 97.2%-99.6%(RSD=1.2%,n=6). CONCLUSIONS:The standard can be used for the quality control of Tongmai pill.
ABSTRACT
To recover and use protein resources in excess sludge and decrease the cost of microbial lipid production, heat-alkaline was used to treat the sludge, and the hydrolysate was preliminarily used as nitrogen source to cultivate Rhodosporidium toruloides AS 2.1389 for lipid accumulation. Firstly, we treated the excess sludge under different alkaline conditions (pH 10, pH 12, pH 13) within the reaction time of 5-10 h to investigate the effect of nitrogen source release. Secondly, we used the sludge hydolysate to culture R. toruloides AS, and test the effect on cell growth. Results showed that treatment of excess sludge at pH 13 for 5 h was the most effective for nitrogen release. However, the hydrolysate obtained at pH 10 (5 h) was the most suitable for culturing R. toruloides AS, and under this condition, the inner-cellular lipid content could reach 35% of the total biomass weight.